2/5/2024
•
Claude Nogues
•
5 min read
Share this post:
Kimialys develops custom bioconjugates for Lateral Flow Assay (LFA), leveraging its patented surface chemistry, K-One®. K-One® optimises the immobilisation of ligands (such as antibody, antigen or protein) on the nanoparticle surface, while fully protecting the remaining surface against non-specific interactions. With this double benefit, Kimialys’ K-One® coating allows LFA developers to reach their target Limit ofDetection (LoD), while maintaining the reliability of their assays, but without requiring any further adjustments to the assay manufacturing parameters. This application note provides a case study of Kimialys’ technology integrated into a rapid diagnostic test targeting an infectious disease protein marker.
The performance of a rapid antigen test in dipstick format (no sample pads or conjugate pads) was compared as a function of the bioconjugates used: latex beads versus Kimialys’ gold nanoparticles, with all other components remaining the same (strip, absorption pad, migration buffer, target antigen, capture antibody). The latex bioconjugates were optimised by a third party through passive adsorption of the capture antibody, while Kimialys’ gold bioconjugates relied on K-One covalent chemistry. The size of the latex beads was not known, while Kimialys’ gold nanoparticles had a diameter of 40nm ± 3nm. All experiments were carried out in the provided migration buffer.
This short study in dipstick format highlights the 3 main advantages of Kimialys’ K-One® surface chemistry applied to LFA:
Gain in sensitivity: K-One® coated gold nanoparticles generate an 8-fold gain in LoD compared to latex beads. This results from optimal orientation of the capture antibodies pointing the recognition site (paratope) towards the solution, thereby maximising capture efficiency.
Background noise removal: No background noise is observed on the strip after the migration of K-One® based bioconjugates, while latex bead migration leads to a faint pink staining. This showcases the optimal protection of the surface brought by K-One® against non-specific interactions.
No R&D transfer cost: switching from latex beads to K-One® gold bioconjugates does not require any change to the other test parameters (membrane, pads, buffers).
Subscribe to our monthly newsletter and stay informed about the latest news, application notes, and insights from our activities.
By clicking Sign Up you're confirming that you agree with our Terms and Conditions.
Join our newsletter to stay up to date on features and releases.
By subscribing you agree to with our Privacy Policy and provide consent to receive updates from our company.
