Products & Applications
Kimialys develops custom bioconjugates for Lateral Flow Assay (LFA), leveraging their patented surface chemistry, K-One. K-One optimises the immobilisation of the ligand (such as antibody, antigen or protein) on the nanoparticle surface, while fully protecting the remaining surface against non-specific interactions.
With this double benefit, Kimialys’ K-One allows LFA developers to reach their target Limit of Detection (LoD), while maintaining the reliability of their assays, but without requiring any further adjustments to the assay manufacturing parameters.
This application note provides a case study of Kimialys’ technology integrated into a rapid diagnostic test targeting an infectious disease protein marker.
Proof of Concept
Here we compare the performance of a rapid antigen test in dipstick format (no sample pads or conjugate pads) as a function of the bioconjugates used: latex beads verses Kimialys’ gold nanoparticles, with all other components remaining the same (strip, absorption pad, migration buffer, target antigen, capture antibody).
The latex bioconjugates were optimized by a third party through passive adsorption of the capture antibody, while Kimialys’ gold bioconjugates relied on K-One covalent chemistry.
The size of the latex beads was not known, while Kimialys’ gold nanoparticles had a diameter of 40nm ± 3nm.
All the experiments were carried out in a migration buffer.
Latex Bioconjugates

- Visual detection around 78 pg/mL
- No false positive observed (no non-specific interaction)
- Background noise after 20 minutes visible as pink staining on membrane
Kimialys’ Bioconjugates


- Visual detection around 10 pg/mL
- No false positive observed (no non-specific interaction) over 12 repetitions with 2 different lots
- No background noise observed after 20 minutes of migration